IL-33 Enhanced the Proliferation and Constitutive Production of IL-13 and IL-5 by Fibrocytes
Figure 2
Effects of IL-33 on fibrocyte proliferation and endogenous TGF-1 production. Fibrocytes were cultured in the presence of various concentrations of IL-33. After 72 h of culture, relative viable cell number and DNA synthesis were determined by a colorimetric cell viability assay (a) and a BrdU incorporation assay (b), respectively. The concentration of TGF-1 in the supernatants was measured by ELISA (c). In (d), the relative proliferation of fibrocytes stimulated with or without 10 ng/mL IL-33 in the presence (closed bars) or absence (open bars) of 20 μg/mL anti-TGF-1 antibody was analyzed by a colorimetric assay. The data are expressed as means SEM (). , n.s.: not significant.