Research Article

IL-33 Enhanced the Proliferation and Constitutive Production of IL-13 and IL-5 by Fibrocytes

Figure 2

Effects of IL-33 on fibrocyte proliferation and endogenous TGF- 1 production. Fibrocytes were cultured in the presence of various concentrations of IL-33. After 72 h of culture, relative viable cell number and DNA synthesis were determined by a colorimetric cell viability assay (a) and a BrdU incorporation assay (b), respectively. The concentration of TGF- 1 in the supernatants was measured by ELISA (c). In (d), the relative proliferation of fibrocytes stimulated with or without 10 ng/mL IL-33 in the presence (closed bars) or absence (open bars) of 20 μg/mL anti-TGF- 1 antibody was analyzed by a colorimetric assay. The data are expressed as means SEM ( ). , n.s.: not significant.
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(d)