IL-33 Enhanced the Proliferation and Constitutive Production of IL-13 and IL-5 by Fibrocytes

Figure 6

Effects of montelukast on cell proliferation, expression of IL-13, and pro-MMP-9 activity. Fibrocytes were cultured with (closed circles and bars) or without (open circles and bars) 10 ng/mL IL-33 for 72 h in the presence of various concentrations (a) and (b) or 10 μM (c) montelukast (MK). Relative proliferation (a), IL-13 production (b), and pro-MMP-9 activity (c) were analyzed by colorimetric cell viability assays, ELISA, and gelatin zymography, respectively. Results are means ± SEM (𝑛=6). ^∗𝑃<0.05 (compared with 0 μM MK), ^#𝑃<0.05, n.s.: not significant.

(a)

(b)

(c)