Store-Operated Ca²⁺ Entry Does Not Control Proliferation in Primary Cultures of Human Metastatic Renal Cellular Carcinoma

Figure 2

Store-operated Ca²⁺ entry is present in primary cultures of mRCC cells. The “Ca²⁺ add-back” protocol revealed that emptying the intracellular Ca²⁺ pool with CPA (10 μM; (a)), thapsigargin (2 μM; (b)), or ionomycin (5 μM; (c)) in the absence of extracellular Ca²⁺ (0Ca²⁺) led to a robust increase in [Ca²⁺]_i upon Ca²⁺ restoration to the bath, which is the hallmark of SOCE. Quantitative real-time reverse transcription polymerase chain reaction of total RNA performed by using specific primers revealed that transcripts encoding for Stim1-2 (d), Orai1–3 (e), and TRPC1–7 (f) are expressed in mRCC cells. Bars represent the mean ± SEM of at least 4 different experiments each from different RNA extracts (see Section 2 for details). ∗ and ^∘𝑃<0.05 (one-way ANOVA followed by Newman-Keuls’ 𝑄 Test).

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(b)

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