Store-Operated Ca2+ Entry Does Not Control Proliferation in Primary Cultures of Human Metastatic Renal Cellular Carcinoma
Figure 8
Stromal derived factor 1-α (SDF-1α), vascular endothelial growth factor (VEGF), and foetal bovine serum (FBS) do not trigger store-operated Ca2+ entry in mRCC cells. (a) fraction of cells responding to SDF-1α in mRCC cells and EPCs. (b) SDF-1α (10 ng/mL), produced a Ca2+ transient (black tracing) in mRCC cells while it elicited a biphasic increase in [Ca2+]i, which is the hallmark of SOCE activation, in EPCs. (c) SOCE was triggered by SDF-1α (10 ng/mL) in EPCs, but not in mRCC cells. (d) 2-APB (50 μM), CAI (10 μM), Gd3+ (100 μM) did not inhibit mRCC cell proliferation. Inset, lower, and higher doses of 2-APB and CAI did not impair mRCC cell proliferation. (e) Brief elevation in [Ca2+]i induced by VEGF (100 ng/mL) in mRCC cells. (f) VEGF (10 ng/mL) ignites repetitive Ca2+ oscillations in EPCs. (g) Fraction of cells responding to VEGF in mRCC cells and EPCs. (h) 20% foetal bovine serum (FBS) elicits a biphasic elevation in [Ca2+]i in EPCs, but not mRCC cells.
