Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets
Table 3
Primers designed in this study for the amplification of the L. monocytogenes virulence genes.
Primer
Sequence 5′-3′
Target
Product (bp)
inlA ext Fw
CGGCTCCGTAGACAGATTAG
inlA
2884
inlA ext Rv
GTGATAGTCTCCGCTTGTAC
inlA In
1-Fw
GTGAGAAGAAAACGA
1200
inlA Detec-Rv
TGGTGTAAGATCGCT
inlA Detec-Fw
AAGTGATATAACTCC
—
inlB ext Fw
GCTAGATGTGGTTTTCGGACT
inlB
2146
inlB ext Rv
TAAGCAGCGCAAAGGTGATTCCTAC
inlB In-Fw
GTGAAAGAAAAGCAC
1227
inlB Seq
3-Rv
ATTCCCGCGAATATA
inlB Seq
2-Fw
TGATGGAACGGTAAT
900
inlB End
3-Rv
TNATTTCTGTGCCCT
plcB ext Fw
CCATACGACGTTAATTCTTGCAATG
plcB
1039
plcB ext Rv
TATCCACCCGCTAACGAGTG
plcA ext Fw
GAGGTTGCTCGGAGATATAC
plcA
1100
plcA ext Rv
CTGCTGTCCCTTTATCGTCG
plcA Detec-Fw
AACCATTATTATGCG
396
plcA Detec-Rv
TGCAGCATACTGACG
hly ext Fw
CGATAAAGGGACAGCAGGACT
hly
1796
hly ext Rv
AGCCTGTTTCTACATTCTTCACAA
hly Detec-Fw
TAACAACGCAGTAAA
566
hly Detec-Rv
CGTAAGTCTCCGAGG
hly End-Fw
CCTCCTGCATATATC
725
hly End-Rv
TTATTCGATTGGATT
inlC In
1-Fw
ATGCTAGTNTTAATTGTA
inlC
852
inlC End
2-Rv
CTAATTCTTGATAGGTTGTG
prfA Detec-Fw
CTGCTAACAGCTGAGCTATG
prfA
404
prfA Detec-Rv
GCTACCGCATACGTTATC
prfA End Rv
ATGAACGCTCAAGCA
—
In: primers corresponding to the beginning of the gene; End: primers corresponding to the end of the gene; Detec: internal primers designed for gene detection; ext: external primers; Seq: internal primers designed for sequencing.
