Research Article

Molecular Identification and Quantification of Tetracycline and Erythromycin Resistance Genes in Spanish and Italian Retail Cheeses

Figure 3

PCR-DGGE analysis of total DNA from Spanish cheeses using specific DGGE-primers for the tetracycline resistance tet(W) gene. Lanes from 1 to 6, Cabrales, Zamorano, Torta del Casar, Ibores, Garrotxa, and De La Mesta. Lanes M, DGGE markers consisting of amplicons obtained with specific DGGE primers for tet(M), tet(O), and tet(W), and using as template total DNA from bacterial strains harboring the respective tetracycline resistance gene. The asterisks denote bands that did no reamplification and, consequently, could not be identified.
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