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BioMed Research International
Volume 2014 (2014), Article ID 748101, 10 pages
http://dx.doi.org/10.1155/2014/748101
Research Article

Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury

1Department of Community Pharmacy, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland
2Department of Clinical Chemistry and Laboratory Diagnostics, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland
3Center of Experimental Medicine, Medical University of Silesia in Katowice, 40-752 Katowice, Poland

Received 2 January 2014; Accepted 3 February 2014; Published 11 March 2014

Academic Editor: Jakub Taradaj

Copyright © 2014 Pawel Olczyk et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of the study was to assess the propolis effect on fibronectin metabolism in the course of burn wounds healing process. A model of burn wound healing of pig skin was applied. The amount of the released glycoprotein was assessed by a surface plasmon resonance. The profile of extracted fibronectin components was also assessed by an electrophoresis in polyacrylamide gel, with a subsequent immunodetection by Western Blotting. Propolis burn treatment decreased the release of fibronectin components from healing wounds in relation to damages treated with silver sulfadiazine. The main reason of decreased extraction of fibronectin components from wounds treated with propolis was a substantial decrease of degradation product release of the mentioned glycoprotein, which was observed particularly from the 3rd to 5th day of the repair. Wounds treatment with propolis demonstrated, especially in relation to damages treated with silver sulfadiazine, the decreased release of synthesized fibronectin molecules. The obtained results suggest that propolis modifies fibronectin metabolism in the course of wound healing process. The influence of propolis is reflected in prevention of fibronectin biosynthesis as well as its degradation in the wound area. The above-mentioned metabolic changes may decrease the risk of complications in the repair wounds process.