BioMed Research International / 2014 / Article / Fig 2

Research Article

Evolutionary Dynamics of rDNA Clusters in Chromosomes of Five Clam Species Belonging to the Family Veneridae (Mollusca, Bivalvia)

Figure 2

Two-color FISH of major rDNA (ITS, digoxigenin, Rhodamine, red) and 5S rDNA (5S, biotin, fluorescein, green) probes to mitotic chromosomes (a), surface spread synaptonemal complexes (b), and released chromatin fibers ((c) and (d)) of Ruditapes decussatus (RDE). Major rDNA red signals are intercalary on the long arms of a submetacentric chromosome pair (bivalent) overlapping 5S rDNA green signals, therefore giving yellow signals. The remaining 5S rDNA signals are terminal to the long arms of a subtelocentric chromosome pair (bivalent). The green and red signals that overlap in mitotic and meiotic chromosomes are clearly distinct on the released chromatin fibers and show an interspersion pattern ((c) and (d)). Scale bars: 5 μm.

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