The Low Chamber Pancreatic Cancer Cells Had Stem-Like Characteristics in Modified Transwell System: Is It a Novel Method to Identify and Enrich Cancer Stem-Like Cells?
The sphere formation from the lower chamber cells. ((a), (b)) Phase-contrast images of the spheres from the lower chamber cells cultured in DMEM-F12 containing 10% FBS from day 1 to day 5 ((a) for day 2, (b) for 5). The size of the sphere increased in a time-dependent manner. ((c), (d)) Phase-contrast images of the cells from the bulk pancreatic cancer cells and the upper chamber cells cultured in DMEM-F12 containing 10% FBS in the fifth day. No spheres can be detected. (c) was the bulk pancreatic cancer cells, while (d) was the upper chamber cells group. Scale bar equal to 50 μm.
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