Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2014, Article ID 760303, 10 pages
http://dx.doi.org/10.1155/2014/760303
Research Article

The Low Chamber Pancreatic Cancer Cells Had Stem-Like Characteristics in Modified Transwell System: Is It a Novel Method to Identify and Enrich Cancer Stem-Like Cells?

1The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China
2Department of Histology and Embryology, Center of Clinical Medicine and Laboratory, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China
3Department of Immunology, Center of Clinical Medicine and Laboratory, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China

Received 19 August 2013; Revised 30 November 2013; Accepted 11 December 2013; Published 10 February 2014

Academic Editor: Yi-Fang Ping

Copyright © 2014 Dongqing Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. K. Singh, C. Hawkins, I. D. Clarke et al., “Identification of human brain tumour initiating cells,” Nature, vol. 432, no. 7015, pp. 396–401, 2004. View at Publisher · View at Google Scholar · View at Scopus
  2. A. T. Collins, P. A. Berry, C. Hyde, M. J. Stower, and N. J. Maitland, “Prospective identification of tumorigenic prostate cancer stem cells,” Cancer Research, vol. 65, no. 23, pp. 10946–10951, 2005. View at Publisher · View at Google Scholar · View at Scopus
  3. L. Ricci-Vitiani, D. G. Lombardi, E. Pilozzi et al., “Identification and expansion of human colon-cancer-initiating cells,” Nature, vol. 445, no. 7123, pp. 111–115, 2007. View at Publisher · View at Google Scholar · View at Scopus
  4. C. Li, D. G. Heidt, P. Dalerba et al., “Identification of pancreatic cancer stem cells,” Cancer Research, vol. 67, no. 3, pp. 1030–1037, 2007. View at Publisher · View at Google Scholar · View at Scopus
  5. K. Sampieri and R. Fodde, “Cancer stem cells and metastasis,” Seminars in Cancer Biology, vol. 22, pp. 187–193, 2012. View at Publisher · View at Google Scholar · View at Scopus
  6. J. E. Visvader and G. J. Lindeman, “Cancer stem cells in solid tumours: accumulating evidence and unresolved questions,” Nature Reviews Cancer, vol. 8, no. 10, pp. 755–768, 2008. View at Publisher · View at Google Scholar · View at Scopus
  7. L. Patrawala, T. Calhoun, R. Schneider-Broussard et al., “Highly purified CD44+ prostate cancer cells from xenograft human tumors are enriched in tumorigenic and metastatic progenitor cells,” Oncogene, vol. 25, no. 12, pp. 1696–1708, 2006. View at Publisher · View at Google Scholar · View at Scopus
  8. M. R. Clay, M. Tabor, J. H. Owen et al., “Single-marker identification of head and neck squamous cell carcinoma cancer stem cells with aldehyde dehydrogenase,” Head and Neck, vol. 32, no. 9, pp. 1195–1201, 2010. View at Publisher · View at Google Scholar · View at Scopus
  9. J. Lee, S. Kotliarova, Y. Kotliarov et al., “Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines,” Cancer Cell, vol. 9, no. 5, pp. 391–403, 2006. View at Publisher · View at Google Scholar · View at Scopus
  10. D. Wang, H. Zhu, Y. Zhu et al., “CD133+CD44+Oct4+Nestin+ stem-like cells isolated from Panc-1 cell line may contribute to multi-resistance and metastasis of pancreatic cancer,” Acta Histochemica, vol. 115, no. 4, pp. 349–356, 2013. View at Publisher · View at Google Scholar
  11. Y. R. Na, S. H. Seok, D. J. Kim et al., “Isolation and characterization of spheroid cells from human malignant melanoma cell line WM-266-4,” Tumor Biology, vol. 30, no. 5-6, pp. 300–309, 2009. View at Publisher · View at Google Scholar · View at Scopus
  12. N. Haraguchi, T. Utsunomiya, H. Inoue et al., “Characterization of a side population of cancer cells from human gastrointestinal system,” Stem Cells, vol. 24, no. 3, pp. 506–513, 2006. View at Publisher · View at Google Scholar · View at Scopus
  13. N. Platet, J.-F. Mayol, F. Berger, F. Hérodin, and D. Wion, “Fluctuation of the SP/non-SP phenotype in the C6 glioma cell line,” FEBS Letters, vol. 581, no. 7, pp. 1435–1440, 2007. View at Publisher · View at Google Scholar · View at Scopus
  14. G.-M. Shi, Y. Xu, J. Fan et al., “Identification of side population cells in human hepatocellular carcinoma cell lines with stepwise metastatic potentials,” Journal of Cancer Research and Clinical Oncology, vol. 134, no. 11, pp. 1155–1163, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. S. Gibbs, S. Spiekstra, E. Corsini, J. McLeod, and J. Reinders, “Dendritic cell migration assay: a potential prediction model for identification of contact allergens,” Toxicology in Vitro, vol. 27, no. 3, pp. 1170–1179, 2013. View at Publisher · View at Google Scholar
  16. C. D. Logsdon, D. M. Simeone, C. Binkley et al., “Molecular profiling of pancreatic adenocarcinoma and chronic pancreatitis identifies multiple genes differentially regulated in pancreatic cancer,” Cancer Research, vol. 63, no. 10, pp. 2649–2657, 2003. View at Google Scholar
  17. V. Vaccaro, E. Bria, I. Sperduti et al., “First-line erlotinib and fixed dose-rate gemcitabine for advanced pancreatic cancer,” World Journal of Gastroenterology, vol. 19, no. 28, pp. 4511–4519, 2013. View at Publisher · View at Google Scholar
  18. Y. J. Song, S. S. Zhang, X. L. Guo et al., “Autophagy contributes to the survival of CD133+ liver cancer stem cells in the hypoxic and nutrient-deprived tumor microenvironment,” Cancer Letters, vol. 339, no. 1, pp. 70–81, 2013. View at Publisher · View at Google Scholar
  19. A. Saleem, A. Jackson, S. Mukherjee et al., “Radiotherapy in the management of unresectable locally advanced pancreatic cancer: a survey of the current UK practice of clinical oncologists,” Clinical Oncology, vol. 22, no. 4, pp. 257–260, 2010. View at Publisher · View at Google Scholar · View at Scopus
  20. J. Wang, W. Zhang, L. Sun et al., “Green tea drinking and risk of pancreatic cancer: a large-scale, population-based case-control study in urban Shanghai,” Cancer Epidemiology, vol. 36, no. 6, pp. e354–e358, 2012. View at Google Scholar
  21. C. Belli, S. Cereda, S. Anand, and M. Reni, “Neoadjuvant therapy in resectable pancreatic cancer: a critical review,” Cancer Treatment Reviews, vol. 39, no. 5, pp. 518–524, 2013. View at Publisher · View at Google Scholar
  22. D. Fang, T. K. Nguyen, K. Leishear et al., “A tumorigenic subpopulation with stem cell properties in melanomas,” Cancer Research, vol. 65, no. 20, pp. 9328–9337, 2005. View at Publisher · View at Google Scholar · View at Scopus
  23. P. C. Hermann, S. L. Huber, T. Herrler et al., “Distinct populations of cancer stem cells determine tumor growth and metastatic activity in human pancreatic cancer,” Cell Stem Cell, vol. 1, no. 3, pp. 313–323, 2007. View at Publisher · View at Google Scholar · View at Scopus
  24. A. Eramo, F. Lotti, G. Sette et al., “Identification and expansion of the tumorigenic lung cancer stem cell population,” Cell Death and Differentiation, vol. 15, no. 3, pp. 504–514, 2008. View at Publisher · View at Google Scholar · View at Scopus
  25. L. W. M. M. Terstappen, S. Huang, M. Safford, P. M. Lansdorp, and M. R. Loken, “Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34+CD38 progenitor cells,” Blood, vol. 77, no. 6, pp. 1218–1227, 1991. View at Google Scholar · View at Scopus
  26. K.-L. Chen, F. Pan, H. Jiang et al., “Highly enriched CD133+CD44+ stem-like cells with CD133+CD44high metastatic subset in HCT116 colon cancer cells,” Clinical and Experimental Metastasis, vol. 28, no. 8, pp. 751–763, 2011. View at Publisher · View at Google Scholar · View at Scopus
  27. A. Jaggupilli and E. Elkord, “Significance of CD44 and CD24 as cancer stem cell markers: an enduring ambiguity,” Clinical and Developmental Immunology, vol. 2012, Article ID 708036, 11 pages, 2012. View at Publisher · View at Google Scholar
  28. W.-H. Liu, N.-S. Qian, R. Li, and K.-F. Dou, “Replacing Hoechst33342 with Rhodamine123 in isolation of cancer stem-like cells from the MHCC97 cell line,” Toxicology in Vitro, vol. 24, no. 2, pp. 538–545, 2010. View at Publisher · View at Google Scholar · View at Scopus
  29. D. Adamski, J.-F. Mayol, N. Platet, F. Berger, F. Hérodin, and D. Wion, “Effects of Hoechst 33342 on C2C12 and PC12 cell differentiation,” FEBS Letters, vol. 581, no. 16, pp. 3076–3080, 2007. View at Publisher · View at Google Scholar · View at Scopus
  30. F. Montanaro, K. Liadaki, J. Schienda, A. Flint, E. Gussoni, and L. M. Kunkel, “Demystifying SP cell purification: viability, yield, and phenotype are defined by isolation parameters,” Experimental Cell Research, vol. 298, no. 1, pp. 144–154, 2004. View at Publisher · View at Google Scholar · View at Scopus
  31. L. Patrawala, T. Calhoun, R. Schneider-Broussard, J. Zhou, K. Claypool, and D. G. Tang, “Side population is enriched in tumorigenic, stem-like cancer cells, whereas ABCG2+ and ABCG2cancer cells are similarly tumorigenic,” Cancer Research, vol. 65, no. 14, pp. 6207–6219, 2005. View at Publisher · View at Google Scholar · View at Scopus
  32. S.-C. Yu, Y.-F. Ping, L. Yi et al., “Isolation and characterization of cancer stem cells from a human glioblastoma cell line U87,” Cancer Letters, vol. 265, no. 1, pp. 124–134, 2008. View at Publisher · View at Google Scholar · View at Scopus
  33. D.-Y. Hueng, H.-K. Sytwu, S.-M. Huang, C. Chang, and H.-I. Ma, “Isolation and characterization of tumor stem-like cells from human meningiomas,” Journal of Neuro-Oncology, vol. 104, no. 1, pp. 45–53, 2011. View at Publisher · View at Google Scholar · View at Scopus
  34. M. Herreros-Villanueva, J. Zhang, A. Koenig et al., “SOX2 promotes dedifferentiation and imparts stem cell-like features to pancreatic cancer cells,” Oncogenesis, vol. 2, no. 8, p. e61, 2013. View at Publisher · View at Google Scholar
  35. K. Wen, Z. Fu, X. Wu, J. Feng, W. Chen, and J. Qian, “Oct-4 is required for an antiapoptotic behavior of chemoresistant colorectal cancer cells enriched for cancer stem cells: effects associated with STAT3/Survivin,” Cancer Letters, vol. 333, no. 1, pp. 56–65, 2013. View at Publisher · View at Google Scholar
  36. J. D. Holland, A. Klaus, A. N. Garratt, and W. Birchmeier, “Wnt signaling in stem and cancer stem cells,” Current Opinion in Cell Biology, vol. 25, no. 2, pp. 254–264, 2013. View at Publisher · View at Google Scholar
  37. A. Androutsellis-Theotokis, R. R. Leker, F. Soldner et al., “Notch signalling regulates stem cell numbers in vitro and in vivo,” Nature, vol. 442, no. 7104, pp. 823–826, 2006. View at Publisher · View at Google Scholar · View at Scopus
  38. S. Liu, G. Dontu, I. D. Mantle et al., “Hedgehog signaling and Bmi-1 regulate self-renewal of normal and malignant human mammary stem cells,” Cancer Research, vol. 66, no. 12, pp. 6063–6071, 2006. View at Publisher · View at Google Scholar · View at Scopus
  39. C. Karamboulas and L. Ailles, “Developmental signaling pathways in cancer stem cells of solid tumors,” Biochimica et Biophysica Acta, vol. 1830, no. 2, pp. 2481–2495, 2013. View at Publisher · View at Google Scholar