The Study of the Frequency Effect of Dynamic Compressive Loading on Primary Articular Chondrocyte Functions Using a Microcell Culture System

Figure 3

Microscopic observation of cell viability after 5 days of cell culture under different compressive stimulation conditions (strain magnitudes: 20% and 40%; stimulating frequencies: 0.5, 1.0, and 2.0 Hz) using the live/dead fluorescent dye. Green and red dots represent live and dead cells, respectively. The upper and lower images show the cells at the surface and the bottom of the 3D cell culture constructs, respectively.