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BioMed Research International
Volume 2014, Article ID 765130, 5 pages
http://dx.doi.org/10.1155/2014/765130
Research Article

Comparison of Two Analytical Platforms for CSF Biomarkers of Alzheimer’s Disease

1Department of Neurology, University General Hospital of Alicante, Avenida Pintor Baeza 12, 8a C, 03010 Alicante, Spain
2Laboratory of Immunology, University General Hospital of Alicante, Avenida Pintor Baeza 12, 8a C, 03010 Alicante, Spain
3Preventive Medicine Service, University General Hospital of Alicante, Avenida Pintor Baeza 12, 8a C, 03010 Alicante, Spain

Received 10 February 2014; Revised 13 April 2014; Accepted 7 May 2014; Published 29 May 2014

Academic Editor: Hanna Rosenmann

Copyright © 2014 Jose Antonio Monge-Argilés et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cerebrospinal fluid (CSF) biomarkers of Alzheimer’s disease (AD) are currently being assessed with two different assays. Our objective was to study if there is a correlation between values obtained by both techniques, to compare their validity and search for conversion factor between values obtained for every protein. We compared the performances of two commonly used platforms, an enzyme-linked immunosorbent assay (ELISA) and a multiplex (xMAP) technology for measurement of CSF , total tau (T-tau), and phosphorylated tau 181 ( ) proteins, in 30 AD patients and 28 control subjects. The relations between the variables of both techniques were evaluated using the Spearman p correlation coefficient ( ). Receiver operating characteristic and area under the curve (AUC) analyses were calculated for the variables of both techniques. The two assays platforms yielded different absolute values for the various analytes, always higher in ELISA. We found some correction factor between values: 2,1- to 3-fold for ; 4,1- to 4,6-fold for T-tau; and 1,4- to 1,6-fold for . In addition, those values were highly correlated ( : , ; T-tau: , ; : , ) and the AUC for the variables showed very similar values. In conclusion, the results obtained with ELISA and xMAP platforms were highly correlated and its validity is very similar. Differences in absolute values point to the need for a clear description of the technique used. Moreover, we found some conversion factor between values of every protein that may be useful for transformation between both techniques.