Cardiac Phosphoproteomics during Remote Ischemic Preconditioning: A Role for the Sarcomeric Z-Disk Proteins

Figure 2

Quantification of phosphorylated phospholamban (p-PLB) in the same samples measured using Western blotting or TMT tandem mass tagging. Protein expression level for cardiac p-PLB (GAPDH in lower panel) in RIPC (126, 127, and 128) and control (129, 130, and 131) samples are shown in (a). Raw data for identification (c) and quantification (b) of the phosphopeptide RASTIEMPQQAR from PLB using TMT isobaric mass tagging are shown in (b) & (c). The reporter ion cluster from the six different mass tags are shown boxed in panel (c) and expanded in bar chart form in panel (b) for both RIPC (numbers: 126, 127, and 128) and control (129, 130, and 131) samples. The peptide sequence showing the phosphorylated amino acid (Ser 16, underlined) is also shown in panel (b). The samples used in these measurements were taken from series 1.