Research Article

Participation of Integrin α5β1 in the Fibronectin-Mediated Adherence of Enteroaggregative Escherichia coli to Intestinal Cells

Figure 2

Fibronectin/integrin α5β1 complex increases the adhesion of EAEC strain 042. (a) Integrin α5β1- and BSA-coated wells were incubated with increasing concentrations of fibronectin (25, 50, and 100 ng) or (b) with 100 ng of fibronectin (Fn) for 15, 30, or 60 min. Bound fibronectin was detected by ELISA, using anti-fibronectin antibodies. (c) EAEC 042 was added to integrin α5β1- and BSA-coated wells incubated or not with fibronectin. (d) EAEC strain 042 and 042aafA were added to integrin α5β1- and BSA-coated wells incubated with fibronectin. EAEC binding was detected by ELISA using anti-O44 serum. The bars represent the mean for three experiments, with the error bars indicating standard deviation. different between treatments ().
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