Participation of Integrin α5β1 in the Fibronectin-Mediated Adherence of Enteroaggregative Escherichia coli to Intestinal Cells
Figure 3
AafA binding to fibronectin/integrin α5β1 complex. (a) AafA-dsc protein was added to integrin α5β1- and BSA-coated wells incubated or not with fibronectin (Fn). AafA-dsc binding was detected by ELISA using anti-AafA antibodies. The bars represent the mean for three experiments, with the error bars indicating standard deviation. different between treatments (). (b) AafA-dsc and fibronectin or (c) AafA-dsc, fibronectin, and integrin α5β1 were mixed and the complex formed was added to control column or a column with anti-AafA antibodies for coimmunoprecipitation analysis. The eluted fraction obtained from control column (E1 and E3) or a column with anti-AafA antibodies (E2 and E4) was analyzed by Dot blot, using anti-AafA, anti-integrin α5, and anti-fibronectin antibodies. The resulting autoradiography was scanned and signal intensity was quantified by UN-SCAN-IT 6.1 software. One representative experiment of three independent experiments with similar result is shown.
