BioMed Research International / 2014 / Article / Fig 4

Research Article

Angiogenin Expression during Early Human Placental Development; Association with Blood Vessel Formation

Figure 4

Angiogenin immunoreactivity in chorionic villi from human first-trimester placentas. Frozen sections of placentas at week 8 to 9 were reacted with either a monoclonal antibody against angiogenin (a, mANG in green) or a polyclonal antibody purified by affinity on immobilised angiogenin (c, e, f, and pANG in red). Bound antibodies were detected with fluorescent secondary antibodies. Angiogenin immunoreactivity (pANG, in red) was detected in the trophoblastic layer immunostained for cytokeratin 7 (CK7, in green) (e, f), in some cells in the close vicinity of the layer (arrowhead: a, in green; c, e, and f, in red) and in isolated cell masses (VCA), located deeper in the villous stroma (a, in green; c and e, in red). The signal was stronger in some cytotrophoblasts (arrow: a). No signal was observed on cryosections reacted with either nonspecific mouse IgM (control, b) or nonspecific rabbit IgG (control, d). Nuclei were counterstained with Dapi, in blue. Bar, 20 μm. IS: intervillous space; TB: trophoblast layer; VCA: vascular cell aggregate; and VM: villous mesenchyme.