BioMed Research International / 2014 / Article / Tab 1

Research Article

Angiogenin Expression during Early Human Placental Development; Association with Blood Vessel Formation

Table 1

Antibodies used in this study.

Antibody specificitySpecies, isotypeConcentration usedCell specificity in placentaSource

AngiogeninRabbit IgG
Mouse IgM
Clone MANG-1
6 μg/mL
  
6 μg/mL
Aim of the study [10]
  
Bachem (San Carlos, CA)
Cytokeratin 7Mouse IgG1
Clone OV-TL 12/30
2 μg/mLTrophoblastic cellsDako (Glostrup, Denmark)
Pan cytokeratin (5, 6, 8, 17, and probably 19)Mouse IgG1
Clone MNF116
0.9 μg/mLEpithelial glandular cells Trophoblastic cellsDako
VimentinMouse IgG1
Clone V9
5 μg/mLEndothelial cells, mesenchymal cells, and vascular smooth muscle cells
Monocytes
Macrophages
Immunotech (Marseille, France)
Alpha smooth muscle actinMouse IgG2a
Clone 1A4
Ascites fluid
1/300
Vascular smooth muscle cellsSigma ImmunoChemicals (St. Louis, MO)
Ki-67 Mouse IgG1
Clone MIB-1
4 μg/mLProliferating cellsImmunotech
CD31, PECAM-1 (Platelet/endothelial cell adhesion molecule-1 )Mouse IgG1
Clone JC/70A
4 μg/mLEndothelial cellsDako
CD34Mouse IgG1
Clone Qbend 10
4 μg/mLEndothelial cells
Hematopoietic precursors
Immunotech
Tie-2 (Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains-2)Rabbit IgG6 μg/mLEndothelial cellsSanta Cruz Biotechnology (Santa Cruz, CA)
VE-cadherin
(Vascular endothelial cadherin),
CD144, cadherin 5
Mouse IgG1
Clone TEA1/31
4 μg/mLEndothelial cellsImmunotech
VEGF-R2 (Flk-1) (Vascular endothelial growth factor receptor-2)Mouse IgG1
Clone A3
4 μg/mLEndothelial cellsSanta Cruz Biotechnology
vWF (von Willebrand factor)Mouse IgG14 μg/mLEndothelial cellsRoche Diagnostics (Meylan, France)
Epo-R (Erythropoietin receptor)Rabbit IgG6 μg/mLEndothelial cells
Erythroid precursors
Santa Cruz Biotechnology
CD14Mouse IgG2b
Clone MY4
6 μg/mLMature monocytes
Macrophages
Immunotech
CD45 (Leukocyte common antigen)Mouse IgG1
Clone HI30
6 μg/mLLeukocytesBD Pharmingen (Le Pont-de-Claix, France)
Isotype controlMouse IgG1NonspecificCoulter Immunology (Hialeah, FL)
Mouse IgG2aImmunotech
Mouse IgG2bCoulter Immunology
Mouse IgMCoulter Immunology
Rabbit IgGJackson ImmunoResearch Laboratories, Inc (West Grove, PA)
FITC-conjugated donkey anti-mouse IgGAffinity Pure donkey IgG3.5 μg/mLJackson ImmunoResearch
Texas Red-conjugated goat anti-mouse IgG (subclasses 1 + 2a + 2b + 3)Affinity Pure goat IgG, Fc fragment specific3.5 μg/mLJackson ImmunoResearch
FITC-conjugated goat anti-mouse IgMAffinity Pure goat IgG, μ chain specific3.75 μg/mLJackson ImmunoResearch
FITC-conjugated goat anti-rabbit IgGAffinity Pure goat IgG, Fc fragment specific3.75 μg/mLJackson ImmunoResearch
Rhodamine (TRITC-) labelled goat anti-rabbit IgGAffinity Pure goat IgG, absorbed against human IgG4.4 μg/mLSigma BioSciences
Texas Red-conjugated donkey anti-rabbit IgGAffinity Pure donkey IgG3.5 μg/mLJackson ImmunoResearch