Research Article

SIRT1 Inhibition Affects Angiogenic Properties of Human MSCs

Figure 2

Effects of SIRT1 inhibition on proliferation, cell cycle, and apoptosis. (a) hMSCs were treated with sirtinol 100 μM (black bars) or equivalent concentration of DMSO (white bars) for 24, 48, and 72 h. (b) sh-Sirt1 infected hMSCs were plated and proliferation was measured for 24, 48, and 72 h. Each histogram indicates the RLUs# related to cell growth measured at different times. Error bars represent SD of . denotes statistical differences when is compared to control. (c) hMSCs were treated with sirtinol 100 μM or equivalent concentration of DMSO for 24, 48, and 72 h. The cells stained with propidium iodide (PI) were subjected to flow cytometric analysis to determine the cell distributions at each phase of the cell cycle. #RLUs are relative light units (RLUs) related to ATP cellular level.
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