SIRT1 Inhibition Affects Angiogenic Properties of Human MSCs
Effects of sirtinol on SIRT1 expression and HIF-1α. (a) hMSCs were treated with sirtinol (100 μM) or equivalent concentration of DMSO (CTR) for 24 h and then exposed to 1% O2 for 6 h. All cells lysates were analyzed for SIRT1, HIF-1α, and tubulin by Western blot. (b) The amounts of SIRT1, VEGF, and GLUT1 mRNA of hMSCs treated with sirtinol at concentration of 100 μM (black bars) or equivalent concentration of DMSO (white bars) for 24 h and exposed to 1% O2 (H) for 6 h were quantified with real-time PCR. The levels of SIRT1, VEGF, and GLUT1 mRNA of hMSCs exposed to 21% O2 (N) for 24 h were measured. indicates statistical significance from control, .