Research Article

Obligatory Role for Endothelial Heparan Sulphate Proteoglycans and Caveolae Internalization in Catestatin-Dependent eNOS Activation

Figure 1

CST stimulates a PI3K-proteoglycan dependent endocytotic machinery in BAE-1 cells. BAE-1 cells incubated with the water-soluble styryl pyridinium membrane dye (FM 1-43). Pseudocolor images better show the fluorescence intensity increase correlated with the rise in vesicles formation consequent to stimulation with CST 5 nM. In CST + H:ase and CST + Wm samples fluorescence intensity was comparable to control levels. Surface plots close to each image display 3D graphs of pixels intensities in a pseudocolor image. The height and the color represent the pixel intensity.
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