Biological Characterization and Pluripotent Identification of Sheep Dermis-Derived Mesenchymal Stem/Progenitor Cells
Figure 1
Morphology of DMS/PCs in vitro and optimization of DMS/PCs culture condition. (a) DMS/PCs of first three passages are inhomogenous. Afterwards, DMS/PCs were homogenous and the shape was spindle. (A) Primary cells after culture for 48 h. ((B)–(K)) Cell morphologies at passage 0, passage 5, passage 10, passage 15, passage 20, passage 25, passage 30, passage 35, and passage 40 before passage. (L) Cell morphologies at passage 48 (bar = 100 μm). (b) ((A), (B), and (C)) Passage 3 DMS/PCs cultured in systems I, II, and III (bar = 100 μm). The result showed that there was no significant difference between culture systems I and II on the cell vigour (), and for system III, there was significant difference compared to culture systems I and II (). The above results demonstrated that bFGF and EGF could maintain the self-renewing of DMS/PCs, and culture system III is optimal for proliferation of DMS/PCs.
