Biological Characterization and Pluripotent Identification of Sheep Dermis-Derived Mesenchymal Stem/Progenitor Cells
Figure 7
Osteogenic differentiation of DMS/PCs. (a) DMS/PCs morphology and staining detection of the induced group and the control group. ((B), (D), and (F)) The control group of osteogenic differentiation. After 1 d, 7 d, and 14 d, the cells cultured in complete medium had no difference in the morphology and phenotype and were also negative for alizarin red staining. (A) DMS/PCs induced in the inducing culture medium after 1 d, and there is no difference in the morphology and phenotype. (C) The induced group of osteogenic differentiation. After induction for 7 days, the cells became confluent and formed mineralized nodules. (E) The cells of induced group were positive for alizarin red staining (bar = 100 μm). (b) RT-PCR detection of the osteogenic markers OPN, COLI, and ALP expression in induced group and control group. Induced cells were positive for OPN, COLI, and ALP, but the control cells were not. 1: GAPDH; 2: OPN; 3: COLI; 4: ALP.
