Optimization of Milk-Based Medium for Efficient Cultivation of Bifidobacterium pseudocatenulatum G4 Using Face-Centered Central Composite-Response Surface Methodology

Table 1

The 2³ full factorial design and responses for screening experiments.

Run	Factors			Response
	Skim milk (% wt/vol)	Yeast extract (% wt/vol)	Glucose (% wt/vol)	Maximum biomass (log10 CFU per milliliter)
1	2 (−1)	0.5 (−1)	0.5 (−1)	4.495
2	2 (−1)	0.5 (−1)	1.5 (+1)	4.789
3	2 (−1)	3 (+1)	0.5 (−1)	5.335
4	2 (−1)	3 (+1)	1.5 (+1)	5.800
5	4 (0)	1.75 (0)	1 (0)	6.417
6	4 (0)	1.75 (0)	1 (0)	6.671
7	4 (0)	1.75 (0)	1 (0)	6.075
8	4 (0)	1.75 (0)	1 (0)	6.824
9	4 (0)	1.75 (0)	1 (0)	6.874
10	6 (+1)	0.5 (−1)	0.5 (−1)	7.158
11	6 (+1)	0.5 (−1)	1.5 (+1)	7.173
12	6 (+1)	3 (+1)	0.5 (−1)	7.204
13	6 (+1)	3 (+1)	1.5 (+1)	7.638