A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway?

Table 1

The eight unique proteins identified by the higher confidence level (at least three unique peptide sequences matched) with significant difference between CCL-13 and HepG2 cells incubation with chitosan nanoparticles in this study.

Cell	Swiss-Prot Number	Protein Name	Subcellular location	MW (Da)	Score	Queries	pI	Molecular function	Sequence coverage	Peptide
CCL-13	Q16478	Glutamate receptor, ionotropic kainate 5; (GRIK5)	Cell membrane	109195	82	9	8.54	extracellular-glutamate-gated ion channel activity; kainate selective glutamate receptor activity	28%	K.VSTIIIDANASISHLILRK.A + Deamidated (NQ); 2 Phospho (ST); R.LQYLRFASVSLYPSNEDVSLAVSRILK.S + 2 Deamidated (NQ); K.VGPEETPRLQYLR.F; R.LNCNLTQIGGLLDTK.G + 2 Deamidated (NQ); Phospho (ST); R.LNCNLTQIGGLLDTK.G + 3 Deamidated (NQ); Phospho (ST); R.RLNCNLTQIGGLLDTK.G + 2 Deamidated (NQ); Phospho (ST); K.VSTIIIDANASISHLILRK.A + Deamidated (NQ); 2 Phospho (ST); M.PAELLLLLIVAFASPSCQVLSSLR.M + Deamidated (NQ); -.MPAELLLLLIVAFASPSCQVLSSLRMAAILDDQTVCGR.G + Carbamidomethyl (C); Deamidated (NQ); Oxidation (M); 2 Phospho (ST)
	O43182	Rho GTPase-activating protein 6; (ARHGAP6)	Cytoplasm	105882	72	13	7.11	Rho GTPase activator activity; SH3/SH2 adaptor activity; phospholipase activator activity	29%	R.EQQVTQK.K; K.DPGMTGSSGDIFESSSLR.A + 4 Phospho (ST); -.MSAQSLLHSVFSCSSPASSSAASAK.G + Deamidated (NQ); Oxidation (M); 3 Phospho (ST); R.EQQVTQKKLSSANSLPAGEQDSPR.L + 2 Phospho (ST); K.DASDFVASLLPFGNK.R + Deamidated (NQ); Phospho (ST); R.LRSVPIQSLSELERAR.L + Deamidated (NQ); R.LRSVPIQSLSELERAR.L + Phospho (ST); K.MTSLNLATIFGPNLLHKQK.S + Deamidated (NQ); Oxidation (M); K.DPGMTGSSGDIFESSSLR.A + 4 Phospho (ST); R.TQAAAPATEGR.A + Deamidated (NQ); Phospho (ST); R.TQAAAPATEGR.A + Deamidated (NQ); Phospho (ST); K.DPGMTGSSGDIFESSSLR.A + 4 Phospho (ST); R.HSSTDSNKASSGDISPYDNNSPVLSER.S + Deamidated (NQ); 6 Phospho (ST)
HepG2	P05067	Amyloid beta A4 protein; (APP)	Membrane	86888	49	4	4.73	DNA binding; heparin binding; peptidase activator activity; serine-type endopeptidase inhibitor activity; transition metal ion binding	19%	R.LNMHMNVQNGK.W + Deamidated (NQ); 2 Oxidation (M); K.WDSDPSGTKTCIDTK.E + 5 Phospho (ST); K.GAIIGLMVGGVVIATVIVITLVMLKK.K + Oxidation (M); Phospho (ST); R.ALEVPTDGNAGLLAEPQIAMFCGR.L + 2 Deamidated (NQ); Oxidation (M); Phospho (ST)
	P51685	C-C chemokine receptor type 8; (CCR8)	Cell membrane	40817	47	4	8.66	C-C chemokine receptor activity; chemokine receptor activity; coreceptor activity	12%	R.ESCEKSSSCQQHSSR.S + Carbamidomethyl (C); Deamidated (NQ); 3 Phospho (ST); R.ESCEKSSSCQQHSSR.S + Carbamidomethyl (C); 2 Deamidated (NQ); 5 Phospho (ST); R.ESCEKSSSCQQHSSR.S + Carbamidomethyl (C); 2 Deamidated (NQ); 5 Phospho (ST); R.ESCEKSSSCQQHSSR.S + Carbamidomethyl (C); 2 Deamidated (NQ); 5 Phospho (ST)
	P16070	CD44 antigen; (CD44)	Membrane	81487	60	3	5.13	collagen binding; hyaluronic acid binding; hyalurononglucosaminidase activity	4%	R.YGFIEGHVVIPR.I; K.ALSIGFETCRYG FIEGHVVIPR.I; K.EQWFGNRWHEGYR.Q
	O95067	G2/mitotic-specific cyclin-B2; (CCNB2)		45253	58	3	9.21		16%	R.KKLQLVGITALLLASK.Y; K.VPVQPTKTTNVNKQLKPTASVKPVQMEK.L + Deamidated (NQ); Oxidation (M); Phospho (ST); K.AQNTKVPVQPTKTTNVNK.Q + 3 Deamidated (NQ); 2 Phospho (ST)
	Q09666	Neuroblast differentiation-associated protein; (AHNAK)	Nucleus	628699	39	7	5.8		12%	K.GPKFKIPEMHLK.A; K.FKMPSMNIQTHK.I + Deamidated (NQ); Oxidation (M); Phospho (ST); K.MDAEVPDVNIEGPDAKLK.G + Deamidated (NQ); K.KSRLSSSSSNDSGNK.V + 2 Deamidated (NQ); 6 Phospho (ST); K.MKLPQFGISTPGSDLHVNAK.G; K.VHAPGLNLSGVGGKMQVGGDGVK.V + Deamidated (NQ); Oxidation (M); Phospho (ST); R.AGAISASGPELQGAGHSKLQVTMPGIKVGGSGVNVNAK.G + 2 Deamidated (NQ); Oxidation (M); 4 Phospho (ST)
	P09486	Osteonectin/; SPARC protein; (SPARC)	Secreted	34610	89	3	4.73	calcium ion binding; collagen binding; extracellular matrix binding	4%	R.LEAGDHPVELLAR.D; K.HGKVCELDENNTPMCVCQDPTSCPAPIGEFEK.V + 3 Carbamidomethyl (C); R.APLIPMEHCTTR.F