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BioMed Research International
Volume 2014 (2014), Article ID 802539, 7 pages
Research Article

Evidence for the Use of Isoflurane as a Replacement for Chloral Hydrate Anesthesia in Experimental Stroke: An Ethical Issue

1EA 1046-Département de Pharmacologie Médicale, Faculté de Médecine, CHU Lille, 1 Place de Verdun, 59045 Lille Cedex, France
2UDSL, 59000 Lille, France
3IUT A, Université de Lille 1, 59653 Villeneuve d’Ascq Cedex, France
4Université d’Artois, 62307 Lens, France

Received 21 October 2013; Accepted 7 January 2014; Published 27 February 2014

Academic Editor: Ahmet Eroglu

Copyright © 2014 Pétrault Maud et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Since an ethical issue has been raised regarding the use of the well-known anesthetic agent chloral hydrate, owing to its mutagenic and carcinogenic effects in animals, attention of neuroscientists has turned to finding out an alternative agent able to meet not only potency, safety, and analgesic efficacy, but also reduced neuroprotective effect for stroke research. The aim of this study was to compare the potential of chloral hydrate and isoflurane for both modulating the action of the experimental neuroprotectant MK801 and exerting analgesia. After middle cerebral artery occlusion in rats, no difference was observed in 24 h survival rate, success of ischemia, or infarct volume reduction between both anesthetics. However, isoflurane exerted a more pronounced analgesic effect than chloral hydrate as evidenced by formalin test 3 hours after anesthesia onset, thus encouraging the use of isoflurane in experimental stroke models.