Integrated Autofluorescence Characterization of a Modified-Diet Liver Model with Accumulation of Lipids and Oxidative Stress
Figure 7
Examples of intratissue distribution patterns of vitamin A, evidenced in terms of yellow reflection of metallic gold precipitate by means of dark-field microscopy. The gold precipitate is present along the sinusoids, within presumed stellate cells and in the cytoplasm of hepatocytes in control (a), or at the periphery of large lipid droplets and in hepatocyte bile canaliculi in MCD diet, 2 weeks (b). In (b), a whitish reflection from the connective tissue allows identifying blood vessels and the bile duct in the portal region. Bar: 50 μm.
