Characterization of a Mycobacterium avium subsp. avium Operon Associated with Virulence and Drug Detoxification

Figure 1

Mutation of the lprG gene of Mycobacterium avium strain D4ER. Genomic region with flanking genes of lprG gene and insertion of hygromycin (Hyg) resistant cassette is indicated. Arrows above represent the length of lprG and flanking genes in M. avium (sequenced strain 104). Arrows at the bottom indicate expected bands after digestion of M. avium lprG mutant with restriction enzymes. Values between arrows indicate the molecular weight of genes or expected bands, expressed in base pairs (bp).