Characterization of a Mycobacterium avium subsp. avium Operon Associated with Virulence and Drug Detoxification

Table 1

Primer sequences, annealing temperatures (T_a), and sizes of amplified products.

Name	Primer sequence Forward	Primer sequence Reverse	Ta (°C)	Size (bp)
lprGup	ctgagatctggacaccgtcagcgagatgc	gtcaagcttgagggtcagggatgcgaaaa	63	613
lprGdown	gtctctagaggccgcgaaaccatcaac	tgtcttaagcgccagtcgtggaacaggaag	63	754
lprG-0	tcctggacccacacggtgct	gggctccaagcagagcggtg	60	510
IS1245	gccgccgaaacgatctac	aggtggcgtcgaggaagac	62	427
lprG-1	ctatcggccgttttcgcatc	acgctcttgacgttcttggt	58	146
lprG-2	ctgagcatccagggcaagat	ctgaccgttgatggtttcgc	58	309
p55-1	cgatgtacctgctgggctac	atgaagatcccgtacagcgg	58	325
p55-2	gtgttctggatcaacgtgcc	gcgatcaagaaccacagcag	58	443
gapdh 1	ccgtcgtacttgtcgtcgtt	ggctcacctgctgaaattcg	58	306
gapdh 2	cgcgtcgtagtacttcagga	cgtcaacgacgacaagtacg	58	443
lprG-compl	catatgatgcagacccgccgc	catatgtcacgagctcaccgg	61	708
operon-compl	catatgatgcagacccgccgc	catatgctaacgctcacccagcg	62	2,265