Uncoupling Protein 2 Regulates Palmitic Acid-Induced Hepatoma Cell Autophagy
Figure 3
UCP2 overexpression enhanced PA-mediated autophagy. H4IIE cells were transfected with UCP2-bearing plasmid and control vector plasmid and then treated with 250 μM PA for 6h, with or without CQ (10μM). (a) The level of UCP2 mRNA was normalized to 18 s, and UCP2 protein was normalized to -actin. This ratio was set to 100% in the control of BSA. (b) The level of LC3 protein was normalized to -actin, and this ratio was set to 100% in the control of BSA. (c) H4IIE cells were treated with 250μM PA for 6 h. Then LC3 puncta formation was observed using an inverted fluorescence microscope. The numbers of LC3 puncta/cell were counted from ≥100 cells. (d) Cells were treated with PA (250μM) for 6 h, with or without CQ before being processed; then electron microscope was performed at 40,000x magnification. Data are expressed as the mean ± SD for each experiment. All data presented are representative of three separate experiments with consistent results.
