Uncoupling Protein 2 Regulates Palmitic Acid-Induced Hepatoma Cell Autophagy
Figure 5
PA-mediated intracellular ROS production is UCP2 mediated. (a) H4IIE cells were transfected with UCP2 plasmid (UCP2-Tr) or control vector (Vector-Tr). Intracellular ROS production induced by 250 μM PA for 6 h was assessed using DCFH-DA and analyzed by flow cytometry. (b) H4IIE cells were transfected with UCP2 siRNA (UCP2-siRNA) or scrambled siRNA (Scramble) for 72 h to inhibit the expression of UCP2 and treated with PA. (c) H4IIE cells were treated with 50 μM genipin for 24 h, followed by PA, with or without CQ (10 μM). (d)–(f) Intracellular ROS was assessed using DCFH-DA staining and microscopy. Data are expressed as the mean ± SD for each experiment. All data presented are representative of three separate experiments with consistent results.
