Uncoupling Protein 2 Regulates Palmitic Acid-Induced Hepatoma Cell Autophagy
Figure 7
UCP2 has an antiapoptotic effect in PA-induced apoptosis. H4IIE cells were transfected with UCP2-bearing plasmid and UCP2-siRNA and then treated with 250 μM PA for 24 h. (a) Cellular apoptosis induced by 250 μM PA was analyzed by flow cytometry. Data are expressed as the mean ± SD for each experiment. (b) TUNEL-positive cells that were green under fluorescence microscopy were quantified from 100 cells at ×400 magnifications. Data were expressed as the mean ± SD for each experiment. (c) Representative of cleaved caspase-3 and -actin proteins expression by western blot. Data were expressed as the mean ± SD. All data presented are representative of three separate experiments with consistent results.
