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BioMed Research International
Volume 2014, Article ID 810401, 14 pages
http://dx.doi.org/10.1155/2014/810401
Research Article

Uncoupling Protein 2 Regulates Palmitic Acid-Induced Hepatoma Cell Autophagy

Department of Pathophysiology, Capital Medical University, 10 You An Men Wai Xi Tou Tiao, Beijing 100069, China

Received 10 April 2014; Revised 29 June 2014; Accepted 30 June 2014; Published 4 August 2014

Academic Editor: Patrice Codogno

Copyright © 2014 Jiaxin Lou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplement FIGURE 1: LC3-II level increased by PA in UCP2-Tr cells. UCP2 over-expression H4IIE cells were treated with PA (250 μM) conjugated to fatty acid-free BSA at different time points. H4IIE Cells treated with BSA acted as a control. After the treatment, cell lysates were collected and subjected to western blotting. Data are expressed as the mean ±SD for each experiment. All data presented are representative of three separate experiments with consistent results.

Supplement FIGURE 2: The effects of PA on UCP2 over-expression cell . UCP2 over-expression H4IIE cells were treated with PA (6 h) conjugated to fatty acid-free BSA at different concentrations, or H4IIE cells were treated with PA (250 μM) conjugated to fatty acid-free BSA at different time points. H4IIE Cells treated with BSA acted as a control. After treatments, cells were stained and subjected to the WST-1 assay. Data are expressed as the mean ±SD for each experiment. All data presented are representative of three separate experiments with consistent results.

Supplement FIGURE 3: PA induces UCP2 expression in Brl cells. Brl cells were treated with PA (6 h) conjugated to fatty acid-free BSA or H4IIE cells treated with BSA. Brl Cells treated with BSA acted as a control. After treatments, cells were stained and subjected to the WST-1 assay. Data are expressed as the mean ±SD for each experiment. All data presented are representative of three separate experiments with consistent results.

Supplement FIGURE 4: OA induces autophagy in H4IIE cells. H4IIE cells were treated with OA (250 μM) conjugated to fatty acid-free BSA for (2, 4, 6, 8, 12, and 24 h) as indicated. Cells treated with BSA acted as a control. After the treatment, cell lysates were collected and subjected to western blotting. Data are expressed as the mean ±SD for each experiment. All data presented are representative of three separate experiments with consistent results.

Supplement FIGURE 5: The effects of CQ on H4IIE cell. H4IIE cells were treated with CQ (24 h) conjugated to fatty acid-free BSA for (2.5, 5, 10, 20, and 50 μM) as indicated. Cells treated with BSA acted as a control. After treatments, cells were stained and subjected to the WST-1 assay. Data are expressed as the mean ±SD for each experiment. All data presented are representative of three separate experiments with consistent results.

  1. Supplementary Material