Evaluation of Cytotoxic and Antimicrobial Effects of Two Bt Cry Proteins on a GMO Safety Perspective

Figure 5

Growth curves of the yeast (a) Candida albicans, (b) C. tropicalis, (c) Pichia anomala, and (d) Saccharomyces cerevisiae grown in BHI broth, pH 5.0, containing Cry8Ka5, Cry1Ac, and ovalbumin proteins, all in concentration of 1,000 𝜇g/mL, and nonprotein controls, 0.05 M sodium phosphate buffer, pH 7.0, and 0.4% formol. The coefficients of variation for each point were ≤10%. For all readings Abs₆₀₀, Cry8Ka5, and Cry1Ac showed no significant difference (𝑃>0.05, ANOVA) compared to the controls, ovalbumin, or 50 mM sodium phosphate buffer, pH 7.0.

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