Evaluation of Cytotoxic and Antimicrobial Effects of Two Bt Cry Proteins on a GMO Safety Perspective

Table 2

Presence of hemolytic activity in different mammals’ species erythrocytes suspensions at 2% treated with Cry8Ka5, Cry1Ac, and other protein and nonprotein controls.

Erythrocytes (2%)	Distilled water (positive control)		NaCl 0.9%; (Negative control)		Cry8Ka5; 1,000 𝜇g/mL			Cry1Ac; 1,000 𝜇g/mL			Bovine serum albumin; 1,000 𝜇g/mL
	Abs540^a	% Hem^b	Abs540	% Hem	Abs540	% Hem	IC50^c; 𝜇g/mL	Abs540	% Hem	IC50; 𝜇g/mL	Abs540	% Hem	IC50; 𝜇g/mL
Human blood type
A	0.456	100	0.119	0	0.115	0	>1,000	0.173	11.8	>1,000	0.092	0	>1,000
B	0.675	100	0.107	0	0.110	0.7	>1,000	0.140	4.9	>1,000	0.089	0	>1,000
AB	0.648	100	0.112	0	0.106	0	>1,000	0.220	16.6	>1,000	0.089	0	>1,000
O	0.932	100	0.106	0	0.113	0.75	>1,000	0.150	4.7	>1,000	0.087	0	>1,000
Rabbit	0.681	100	0.109	0	0.107	0	>1,000	0.141	4.6	>1,000	0.096	0	>1,000
Rat	0.446	100	0.143	0	0.126	0	>1,000	0.205	13.9	>1.000	0.126	0	>1,000

The absorbance values (Abs) and % hemolysis are averages of triplicates. The standard deviations were lower than 20%. ^aWavelength (nm) used to measure the release of hemoglobin; ^b% Hemolysis = (Abs₅₄₀ sample − Abs₅₄₀ of the negative control) × 100/Abs₅₄₀ of the positive control; ^cConcentration capable of causing 50% hemolysis of the cells.