Research Article

Age and SPARC Change the Extracellular Matrix Composition of the Left Ventricle

Figure 3

Collagen IV expression levels increased with age in the wild type (WT), but not in the SPARC-null (null) mice. Collagen IV protein levels were quantified by immunoblot ( /genotype/age). Two controls were used, total protein stain (loading control) and molecular weight marker (MW, blot to blot control). The signal intensity of the MW was used to normalize the data among blots, while protein levels were normalized to the total protein in its respective lane. Values were plotted as mean ± SEM; * versus respective young group.
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