Curcumin, a Natural Antioxidant, Acts as a Noncompetitive Inhibitor of Human RNase L in Presence of Its Cofactor 2-5A In Vitro

Figure 5

Structure of human RNAse L (4OAU)-curcumin complex obtained after docking analysis as described in materials and methods. Sites filled with magenta dots are pockets predicted as potential binding sites for curcumin. Site 1 is occupied by 2-5A and site 3 by ADP in native structure. Residues highlighted in blue, cyan, and green are active site residues showing position of active site [21]. Inset Figures 5(a) and 5(b) show interaction of curcumin (backbone C in orange color) at site 4 and site 2, respectively. Oxygen atoms are shown in red and nitrogen in blue. Green dotted lines indicate possible H-bonds. Wireframe spheres are interaction spheres between atoms of ligand and receptor.