Accumulation of Extracellular Hyaluronan by Hyaluronan Synthase 3 Promotes Tumor Growth and Modulates the Pancreatic Cancer Microenvironment
Figure 1
HAS3 overexpression is associated with high extracellular hyaluronan accumulation and HAS2 with increased extracellular and intracellular accumulation of hyaluronan. Amount of secreted, cell surface, and intracellular hyaluronan was analyzed in BxPC-3, BxPC-3 HAS2, and BxPC-3 HAS3 cells with a hyaluronan assay, and results were presented as extracellular hyaluronan showing combined amount of secreted and cell surface hyaluronan (a) and intracellular hyaluronan (b). The results were expressed as % of hyaluronan content in BxPC-3 cells, and data represent mean ± S.D. of three independent experiments. To visualize intracellular hyaluronan, subconfluent cultures of BxPC-3 (c), BxPC-3 HAS2 (d), and BxPC-3 HAS3 cells (e) were stained for intracellular hyaluronan (green) and CD44 (red) using bHABP and anti-CD44s-antibody, respectively. Nuclei were visualized with Prolong Antifade with DAPI. Scale bar in (c–e) is 50 μm. Statistical differences between the groups in (a) and (b) were tested using one-way ANOVA and Tukey’s post hoc test (; ).
