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BioMed Research International
Volume 2014, Article ID 820761, 9 pages
http://dx.doi.org/10.1155/2014/820761
Research Article

Abarema cochliacarpos Extract Decreases the Inflammatory Process and Skeletal Muscle Injury Induced by Bothrops leucurus Venom

1Departamento de Fisiologia, Laboratorio de Farmacologia Pré-Clinica, Universidade Federal de Sergipe, SE, Brazil
2Departamento de Morfologia, Laboratório de Biologia Celular e Estrutura, Universidade Federal de Sergipe, SE, Brazil
3Programa de Pós-Graduação em Biotecnologia (RENORBIO), Universidade Federal de Sergipe, SE, Brazil
4Departamento de Fisiologia, Laboratório de Bioquímica e Química de Produtos Naturais, SE, Brazil
5Laboratório de Farmacologia das Toxinas, ICB, UFRJ, Universidade Federal do Rio de Janeiro, RJ, Brazil
6Instituto Butantan, Laboratório de Ciências Fisiológicas e Química, São Paulo, SP, Brazil
7Departamento de Farmácia, Laboratório de Ensaios e de Toxicidade Farmacêutica, Universidade Federal de Sergipe, SE, Brazil
8Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco, PE, Brazil
9Departamento de Biofísica e Fisiologia, Universidade Federal do Piaui, Teresina, PI, Brazil
10Laboratory Preclinical Pharmacology of Natural Products, Department of Physiology, Federal University of Sergipe, S/N Marechal Rondon Avenue, 49.100-000 São Cristovão, SE, Brazil

Received 26 January 2014; Revised 20 April 2014; Accepted 11 May 2014; Published 20 July 2014

Academic Editor: Stephen E. Alway

Copyright © 2014 Jeison Saturnino-Oliveira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Snakebites are a public health problem, especially in tropical countries. However, treatment with antivenom has limited effectiveness against venoms’ local effects. Here, we investigated the ability of Abarema cochliacarpos hydroethanolic extract (EAc) to protect mice against injection of Bothrops leucurus venom. Swiss mice received perimuscular venom injection and were subsequently treated orally with EAc in different doses. Treatment with EAc 100, 200, and 400 mg/kg reduced the edema induced by B. leucurus in 1%, 13%, and 39%, respectively. Although lower doses showed no antihypernociceptive effect in the Von Frey test, the higher dose significantly reduced hyperalgesia induced by the venom. Antimyotoxic activity of EAc was also observed by microscopy assessment, with treated muscles presenting preserved structures, decreased edema, and inflammatory infiltrate as compared to untreated ones. Finally, on the rotarod test, the treated mice showed better motor function, once muscle fibers were preserved and there were less edema and pain. Treated mice could stand four times more time on the rotating rod than untreated ones. Our results have shown that EAc presented relevant activities against injection of B. leucurus venom in mice, suggesting that it can be considered as an adjuvant in the treatment of envenomation.