IC50/EC50: in antiviral assays, half maximal effective concentration (EC50) refers to the concentration of compound causing 50% reduction of virus replication in cell based assays. Half maximal inhibitory concentration (IC50) is used when virus inhibition is estimated from in vitro inhibition assays, such as the inhibition of a viral enzyme. Sometimes both EC50 and IC50 values are used loosely to describe the same antiviral activity. In this review, we are following the original nomenclature used by each cited paper.
1Viral plaque reduction assay.
2Plaque formation assay.
3Focus forming assay.
4Virus induced CPEs.
7Viral EGFP (enhanced green fluorescent protein) expression.
8Reduction of luciferase activity.
*Tested in vivo in animal model.