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BioMed Research International
Volume 2014, Article ID 828515, 8 pages
Research Article

Effects of Subchronic Exposure to N,N-Diethyl-m-toluamide on Selected Biomarkers in Common Carp (Cyprinus carpio L.)

1Department of Veterinary Public Health and Animal Welfare, University of Veterinary and Pharmaceutical Sciences Brno, Palackého Třída 1/3, 612 42 Brno, Czech Republic
2Department of Immunology, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
3Department of Anatomy, Histology and Embryology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého Třída 1/3, 612 42 Brno, Czech Republic

Received 3 January 2014; Revised 27 February 2014; Accepted 28 February 2014; Published 30 March 2014

Academic Editor: Josef Velíšek

Copyright © 2014 Andrea Slaninova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


DEET (N,N-diethyl-m-toluamide) is the most common active ingredient in the insect repellents commonly detected in European groundwater. The aim of this study was to investigate the effect of subchronic DEET exposure on biochemical and haematological parameters, antioxidant enzymes, including catalase, glutathione peroxidase, glutathione reductase, and glutathione S-transferase, and the amount of thiobarbituric acid reactive substances (TBARS) in common carp (Cyprinus carpio L.). Two specific proinflammatory and anti-inflammatory cytokine genes were selected to assess an immunological status of the fish. Fish were exposed for 28 days to three concentrations of DEET (1.0 µg/L, 0.1 mg/L, and 1.0 mg/L) where 1 µg/L is corresponding to the concentration found in the environment. DEET had a significant effect on increased RBC, decreased mean corpuscular volume (MCV), and mean corpuscular haemoglobin value (MCH) compared to control groups in the concentration of 1 mg/L. A significant decline in triacylglycerols (TAG) in plasma was found in the concentration of 1 mg/L compared to the control groups. The parameters of oxidative stress in tissues of common carp were weekly affected and immunological parameters were not affected.