P2Y Receptor Modulation of ATP Release in the Urothelium
Figure 3
Phosphate liberation from endogenously released ATP. Incubation of RT4 urothelial cells in control or hypotonic phosphate free buffer for 10 or 30 minutes induced ATP release (a). Over the 10- or 30-minute incubation a small amount (approximately 10%) of this ATP was broken down by urothelial cells to liberate phosphate that could be detected (b). The presence of the ectoATPase inhibitor ARL67156 (100 μM) was seen to increase the amount of ATP detected after both 10 and 30 minutes (c).
