Research Article

Repeated Batch Fermentation Biotechnology for the Biosynthesis of Lipid and Gamma-Linolenic Acid by Cunninghamella bainieri 2A1

Table 1

Test variables and levels for central composite design (CCD).

VariableSymbolLevel
Actual rangeCoded value
LowMiddleHighLowMiddleHigh

Glucose concentration (g/L) 203040−10+1
Ammonium tartrate (g/L) 12.54−10+1
Harvesting time (h) 123048−10+1