Repeated Batch Fermentation Biotechnology for the Biosynthesis of Lipid and Gamma-Linolenic Acid by Cunninghamella bainieri 2A1

Table 5

Analysis of variance for the second-order polynomial model of lipid content produced by Cuninghamella bainieri 2A1 using repeated batch cultivation at 30°C for 96 h.

Source	Polynomial coefficients	Sum of squares	DF	Mean square	𝐹 value	Prob > 𝐹
Model		583.88	9	64.88	276.48	<0.0001**
Intercept	21.84
𝑋1	−0.15	0.23	1	0.23	0.96	0.3561
𝑋2	−2.86	81.80	1	81.80	348.59	<0.0001**
𝑋3	6.74	454.28	1	454.28	1936.01	<0.0001**
𝑋^21	0.042	4.704𝐸−003	1	4.704𝐸−003	0.020	0.8909
𝑋^22	1.39	5.25	1	5.25	22.37	0.0015**
𝑋^23	−0.41	0.45	1	0.45	1.93	0.2027
𝑋1𝑋2	0.75	4.50	1	4.50	19.18	0.0024**
𝑋1𝑋3	−0.15	0.18	1	0.18	0.77	0.4066
𝑋2𝑋3	−2.12	36.13	1	36.13	153.96	<0.0001**
Residuals		1.88	8	0.23
Lack of fit		1.69	5	0.34	5.41	0.0977
Pure error		0.19	3	0.063

∗∗Statistically significant at 99% of probability level.
𝑋₁: glucose concentration (g/L); 𝑋₂: ammonium tartrate concentration (g/L); 𝑋₃: harvesting time (h). 𝑋²₁, 𝑋²₂, and 𝑋²₃: the quadratic terms; 𝑋₁𝑋₂, 𝑋₁𝑋₃, and 𝑋₂𝑋₃: the interaction terms.
𝑅²=0.9968.