Repeated Batch Fermentation Biotechnology for the Biosynthesis of Lipid and Gamma-Linolenic Acid by Cunninghamella bainieri 2A1

Table 6

Analysis of variance for the second-order polynomial model of GLA production by Cuninghamella bainieri 2A1 using repeated batch cultivation at 30°C for 96 h.

Source	Polynomial coefficients	Sum of squares	DF	Mean square	𝐹 value	Prob > 𝐹
Model		0.15	9	0.017	8.44	0.0031**
Intercept	0.18
𝑋1	−0.047	0.022	1	0.022	11.05	0.0105*
𝑋2	0.025	6.250𝐸−003	1	6.250𝐸−003	3.13	0.1150
𝑋3	−0.022	4.840𝐸−003	1	4.840𝐸−003	2.42	0.1584
𝑋^21	7.976𝐸−003	1.724𝐸−004	1	1.724𝐸−004	0.086	0.7765
𝑋^22	−0.012	3.917𝐸−004	1	3.917𝐸−004	0.20	0.6698
𝑋^23	0.13	0.048	1	0.048	23.96	0.0012**
𝑋1𝑋2	0.071	0.041	1	0.041	20.31	0.0020**
𝑋1𝑋3	−3.750𝐸−003	1.125𝐸−004	1	1.125𝐸−004	0.056	0.8185
𝑋2𝑋3	−0.014	1.513𝐸−003	1	1.513𝐸−003	0.76	0.4096
Residuals		0.016	8	1.999𝐸−003
Lack of fit		0.014	5	2.824𝐸−003	4.52	0.1222
Pure error		1.875𝐸−003	3	6.250𝐸−004

∗Statistically significant at 95% probability level.
∗∗Statistically significant at 99% of probability level.
𝑋₁: glucose concentration (g/L); 𝑋₂: ammonium tartrate concentration (g/L); 𝑋₃: harvesting time (h). 𝑋²₁, 𝑋²₂, and 𝑋²₃: the quadratic terms; 𝑋₁𝑋₂, 𝑋₁𝑋₃, and 𝑋₂𝑋₃: the interaction terms.
𝑅²=0.9047.