Research Article

Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma

Figure 3

Caveolin-1 and flotillin-1 protein expression in homogenates of HCC and ANT. (a) Western blotting analysis of whole tissue homogenates (25 μg protein/lane) prepared from matched HCC and ANT samples in different histologic grades probed with antibodies to caveolin-1 (left) and flotillin-1 (right) as indicated and simultaneously with antibody to β-actin. (b) Densitometric quantification of bands relative to caveolin-1 (left) and flotillin-1 (right) in HCC and ANT tissue homogenates of 30 patients in each group, after SDS-PAGE immunodetection. Band intensity is normalized to β-actin.
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