BioMed Research International / 2014 / Article / Fig 5

Research Article

Upregulation of Relaxin after Experimental Subarachnoid Hemorrhage in Rabbits

Figure 5

Time course of changes in myosin light chain (MLC) phosphorylation induced by endothelin-1 in human brain vascular smooth muscle cells (HBVSMC). Representative immunoblot analysis of MLC phosphorylation in HBVSMC preincubated without (a) and with (b) human recombinant relaxin-2 (H2 relaxin). The upper, middle, and lower bands detected with anti-MLC antibody represented di-, mono-, and nonphosphorylated forms of MLC, respectively. Summary of the level of MLC phosphorylation (percent of total MLC) induced by 100 nmol/L endothelin-1 at four time points: just before relaxin stimulation (0 minutes) and 5, 10, and 30 minutes after relaxin stimulation ((c) preincubated without H2 relaxin, (d) preincubated with H2 relaxin). The data are the means ± SEM ( ). versus 0 minutes.