Proteomic Analysis of Gossypol Induces Necrosis in Multiple Myeloma Cells
Figure 2
Morphologic images of multiple myeloma cells. (a) Untreated cells; (b) 40 μmol/L gossypol-treated cells for 24 h; all images were captured by Olympus IX2-UCB 60x inverted microscopy; (c) gel electrophoresis of DNA from untreated and gossypol-treated myeloma cells. (d-e) Detection of ROS in untreated and gossypol-treated U266 cells using the Image-iT LIVE Reactive Oxygen Species (ROS) Kit. Cells were labeled with carboxy-H2DCFDA, which exhibited green fluorescence when reacted with ROS, and nuclei were stained with blue-fluorescent Hoechst 33342. (d) Fluorescence image of ROS in untreated U266 cells; and (e) fluorescence image of ROS in 40 μmol/L gossypol-treated cells for 10 h.
