Construction and Characterization of a Bacterial Artificial Chromosome Library for the Hexaploid Wheat Line 92R137
Figure 5
Screening of the BAC library with the Yr26-linked marker Xwe173. (a) Screening of BAC plate pools. A 451-bp band was amplified in positive control 92R137 with Xwe173. Two positive BAC plates, H186 (constructed with HindIII) and B634 (constructed with BamHI), were identified. (b) Six BAC clones (H186H13, H186E15, B634C10, B634C11, B634D10, and B634E10), which were identified from plates H186 and B634, were confirmed to contain Xwe173.
