Research Article

The Trend in Distribution of Q223R Mutation of Leptin Receptor Gene in Amoebic Liver Abscess Patients from North India: A Prospective Study

Figure 1

Schematic representation of the methodology followed for detection of Q223R mutation in leptin receptor gene. Digestion of PCR amplified product of 386 bp by restriction enzyme BseNI (BsrI) yields three bands of in homozygous wild, four bands of in heterozygous, and two bands of in homozygous mutant. Allele “A” codes for glutamine and allele “G” codes for arginine in leptin receptor gene. Lane Marker, lane B. DNA = human blood genomic DNA, lane pus DNA = ALA (amoebic liver abscess) pus DNA, and NTC = no template control.
847132.fig.001