BioMed Research International / 2014 / Article / Fig 2

Research Article

The Trend in Distribution of Q223R Mutation of Leptin Receptor Gene in Amoebic Liver Abscess Patients from North India: A Prospective Study

Figure 2

(a) PCR-RFLP analysis of Q223R SNP (rs1137101) from leptin receptor gene. Lane Marker, lane AG, lane GG, and lane AA represent restriction enzyme digested PCR product of ALA pus DNA of different genotypes. After digestion with BseNI restriction enzyme, wild type AA is visible as two bands 221 bp, 146 bp and the third band of 19 bp is invisible due to smaller size. Heterozygous AG is visible as three bands 367 bp, 221 bp, 146 bp and 19 bp (invisible) whereas homozygous mutant GG is visible as bands of 221 bp, 146 bp and 19 bp (invisible). (b) Sequencing results of wild “A” and mutant “G” are detected by PCR-RFLP confirming the presence of SNP in DNA of ALA patients.

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